abstract：:Measuring the amount of HIV-1 DNA in infected cells is important to estimate the size of the viral reservoir in patients. However, the clinical impact of the intracellular viral DNA level remains unclear. The present study examines the clinical significance of the HIV-1 DNA level in peripheral CD4+ T lymphocytes from ...

journal_title：Microbiology and immunology

authors： Wada K,Nagai H,Hagiwara T,Ibe S,Utsumi M,Kaneda T

更新日期：2004-01-01 00:00:00

abstract：:It has been suggested that infectious entry of rubella virus (RV) is conducted by receptor mediated endocytosis. To explore the cellular entry mechanism of RV, inhibitory effects of drugs affecting various endocytic pathways on RV entry into VeroE6 cells were analyzed. Results showed that RV infectious entry into Vero...

journal_title：Microbiology and immunology

authors： Kee SH,Cho EJ,Song JW,Park KS,Baek LJ,Song KJ

更新日期：2004-01-01 00:00:00

abstract：:Antibodies to influenza A virus were detected using enzyme-linked immunosorbent assay (ELISA) in the sera from two of seven Baikal seals (Phoca sibrica) and from five of six ringed seals (Phoca hispida) in Russia. In a hemagglutination-inhibition test using H1-H15 reference influenza A viruses, ELISA-positive sera fro...

journal_title：Microbiology and immunology

authors： Ohishi K,Kishida N,Ninomiya A,Kida H,Takada Y,Miyazaki N,Boltunov AN,Maruyama T

更新日期：2004-01-01 00:00:00

abstract：:Immune responses to Helicobacter pylori infection play important roles in gastroduodenal diseases. The contributions of tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma) to the induction of gastric inflammation and to the protection from H. pylori infection were investigated using TNF-alpha gene...

journal_title：Microbiology and immunology

authors： Yamamoto T,Kita M,Ohno T,Iwakura Y,Sekikawa K,Imanishi J

更新日期：2004-01-01 00:00:00

abstract：:We examined the mechanisms of fluconazole resistance in a fluconazole-resistant Candida albicans isolate from a Japanese patient with chronic mucocutaneous candidiasis. It was demonstrated that the highly resistant phenotype of this strain was associated with combined mechanisms of the energy-dependent reduced intrace...

journal_title：Microbiology and immunology

authors： Kamai Y,Maebashi K,Kudoh M,Makimura K,Naka W,Uchida K,Yamaguchi H

更新日期：2004-01-01 00:00:00

abstract：:Porphyromonas gingivalis autolyzes in the culture media. To examine in more detail the molecular components of the autolysate, two-dimensional gel electrophoresis was performed. Many protein spots varied both in number and volume. One of these spots included Arg-gingipain (Rgp) as determined by N-terminal amino acid s...

journal_title：Microbiology and immunology

authors： Kamaguchi A,Nakano M,Shoji M,Nakamura R,Sagane Y,Okamoto M,Watanabe T,Ohyama T,Ohta M,Nakayama K

更新日期：2004-01-01 00:00:00

abstract：:Alpha-fetoprotein (AFP) expression is observed in embryonic tissues and, the expression of this protein is absent in normal adult tissues. The re-elevation of serum AFP strongly suggests generation of a malignant tumor in an adult. We demonstrated here that AFP-producing gastric cancer (AFP-gastric cancer) could be tr...

journal_title：Microbiology and immunology

authors： Miura Y,Kataoka H,Joh T,Tada T,Asai K,Nakanishi M,Okada N,Okada H

更新日期：2004-01-01 00:00:00

abstract：:The Wilms' tumor gene WT1 is overexpressed in various types of solid tumors, including lung and breast cancer and WT1 protein is a tumor antigen for these malignancies. In phase I clinical trials of WT1 peptide-based cancer immunotherapy, two patients with advanced lung cancer were intradermally injected with 0.3 mg o...

journal_title：Microbiology and immunology

authors： Tsuboi A,Oka Y,Osaki T,Kumagai T,Tachibana I,Hayashi S,Murakami M,Nakajima H,Elisseeva OA,Fei W,Masuda T,Yasukawa M,Oji Y,Kawakami M,Hosen N,Ikegame K,Yoshihara S,Udaka K,Nakatsuka S,Aozasa K,Kawase I,Sugiyama H

更新日期：2004-01-01 00:00:00

abstract：:gp96 plays a central role in innate as well as acquired immunity, maturation and chemotaxis of dendritic cells, Ab production, and cross-priming, and is a peptide acceptor in endoplasmic reticulum and an accessory to peptide loading of MHC class I molecules. The remarkable conservation of essential immunological prope...

journal_title：Microbiology and immunology

authors： Cho NH,Choi CY,Seong SY

更新日期：2004-01-01 00:00:00

abstract：:For the simple and rapid detection/identification of major pathogenic fungal species such as Candida albicans, C. tropicalis, C. parapsilosis, C. glabrata and Aspergillus fumigatus, common primers for these species and specific primers for each species, designed on the basis on the genomic nucleotide sequences of the ...

journal_title：Microbiology and immunology

authors： Kanbe T,Arishima T,Horii T,Kikuchi A

更新日期：2003-01-01 00:00:00

abstract：:Porphyromonas gingivalis is a pathogen associated with adult periodontitis, which is a chronic inflammatory disease characterized by breakdown of the periodontal tissue. Dipeptidyl aminopeptidase IV (DPPIV) produced by P. gingivalis has been considered to be a potential virulence factor based on the finding that the v...

journal_title：Microbiology and immunology

authors： Kumagai Y,Yajima A,Konishi K

更新日期：2003-01-01 00:00:00

abstract：:Bifidobacteria are important members of the intestinal microbiota and are considered to contribute to maintaining health. However, the level of bifidobacteria colonising the intestine of elderly subjects tends to be lower than in younger adults. Therefore, two Bifidobacterium longum strains, isolated from healthy elde...

journal_title：Microbiology and immunology

authors： Mäkeläinen H,Tahvonen R,Salminen S,Ouwehand AC

更新日期：2003-01-01 00:00:00

abstract：:We examined the role of B-1 cells in protection against Toxoplasma gondii infection using B cell-deficient mice (muMT mice). We found that primed but not naïve B-1 cells from wild-type C57BL/6 mice protected B cell-deficient recipients from challenge infection. All muMT mice transferred with primed B-1 cells survived ...

journal_title：Microbiology and immunology

authors： Chen M,Mun HS,Piao LX,Aosai F,Norose K,Mohamed RM,Belal US,Fang H,Ahmed AK,Kang HK,Matsuzaki G,Kitamura D,Yano A

更新日期：2003-01-01 00:00:00

abstract：:In a previous study, it was found that polyoxotungstates such as undecatungstosilicate (SiW11) greatly sensitized strains of methicillin-resistant Staphylococcus aureus (MRSA) to beta-lactams. In this report, the effects of SiW11 on several MRSA strains with unique resistant mechanisms were studied. SiW11 was still ef...

journal_title：Microbiology and immunology

authors： Tajima Y

更新日期：2003-01-01 00:00:00

abstract：:A 4-year (1996-2000) survey of rotavirus infection involving 2,218 diarrheal fecal specimens of children collected from five regions of Japan was conducted. A total of 642 (28.9%) specimens were found to be rotavirus positive. A changed prevalence pattern of rotavirus G serotype was found with an increase of G9 and G2...

journal_title：Microbiology and immunology

authors： Zhou Y,Li L,Okitsu S,Maneekarn N,Ushijima H

更新日期：2003-01-01 00:00:00

abstract：:There are many examples of probiotic effects of various lactic bacteria on enteropathogens. In this study, Lactobacillus strains (L. rhamnosus, L. gasseri, L. casei and L. plantarum) were tested in an in vitro model of enterohemorrhagic Escherichia coli (EHEC) infection of a human colon epithelial cell line, C2BBe1. W...

journal_title：Microbiology and immunology

authors： Hirano J,Yoshida T,Sugiyama T,Koide N,Mori I,Yokochi T

更新日期：2003-01-01 00:00:00

abstract：:The standard products of V(D)J recombination of immunoglobulin and T cell receptor genes are two kinds of DNA junction, a coding joint and a signal joint. TCR delta V-D and D-D signal joints in adult mouse thymocytes were sequenced following PCR amplification. We observed differential nucleotide insertions at the V de...

journal_title：Microbiology and immunology

authors： Kanari Y,Muto M,Yamagishi H

更新日期：2003-01-01 00:00:00

abstract：:Previously we reported that mice infected recurrently with live Fusobacterim nucleatum (Fn) synthesize a significant amount of NO between 12 hr and 24 hr after the Fn injection. We now investigated whether the NO has the capability of killing Fn, a gram-negative rod periodontal pathogen. The mice were divided into thr...

journal_title：Microbiology and immunology

authors： Kato C,Mikami M,Suzuki A,Saito K

更新日期：2003-01-01 00:00:00

abstract：:VacA, a secretory product of Helicobacter pylori, binds to its cell surface receptor, receptor tyrosine phosphatase (RPTP) beta, leading to cytoplasmic vacuolization of gastric epithelial AZ-521 cells. VacA binding to the cell surface and VacA-dependent vacuolization were inhibited by cell culture media containing fet...

journal_title：Microbiology and immunology

authors： Kimura T,Wada A,Nakayama M,Ogushi K,Nishi Y,De Guzman BB,Moss J,Hirayama T

更新日期：2003-01-01 00:00:00

abstract：:Salmonella enterica serovar Typhi and serovar Paratyphi A with reduced susceptibility to fluoroquinolones (MICs of ciprofloxacin, 0.25 to 2 microg/ml) have a mutation at codon either Ser-83 or Asp-87 of gyrA gene. A screening method by PCR-restriction fragment length polymorphism (PCR-RFLP) was designed to screen the ...

journal_title：Microbiology and immunology

authors： Hirose K,Tamura K,Watanabe H

更新日期：2003-01-01 00:00:00

abstract：:Aged C57BL/6 (B6) mice could reject allogeneic BALB/c RL male 1 tumor as efficiently as young B6 mice. However, in vitro analysis showed impaired generation of cytotoxic T cell response in aged B6 mice against allogeneic tumor. The reaction could be augmented by the addition of recombinant interleukin-2 (rIL-2). Enzym...

journal_title：Microbiology and immunology

authors： Win S,Uenaka A,Nakayama E

更新日期：2002-01-01 00:00:00

abstract：:Seroprevalence of Bartonella henselae was investigated in Japan in 48 individuals clinically suspected of having cat-scratch disease (CSD), 159 patients with cardiovascular diseases, and 129 healthy veterinary students. Of 48 CSD-suspected patients examined, 19 (39.6%) were positive for B. henselae-IgG and 4 (8.3%) fo...

journal_title：Microbiology and immunology

authors： Kikuchi E,Maruyama S,Sakai T,Tanaka S,Yamaguchi F,Hagiwara T,Katsube Y,Mikami T

更新日期：2002-01-01 00:00:00

abstract：:We isolated a filamentous bacteriophage, VfO4K68, from the pandemic Vibrio parahaemolyticus strain belonging to 04:K68 serovar. The VfO4K68 DNA lacked a 1,893-bp fragment present in that of the distinctive region of f237, a filamentous phage isolated from a pandemic 03:K6 strain (Nasu, H. et al., J. Clin. Microbiol., ...

journal_title：Microbiology and immunology

authors： Chan B,Miyamoto H,Taniguchi H,Yoshida S

更新日期：2002-01-01 00:00:00

abstract：:Neonatal toxic shock syndrome-like exanthematous disease (NTED) is a new entity of methicillin-resistant Staphylococcus aureus (MRSA) infection. Most of NTED cases reported previously in the literature were sporadic ones. In the present report, we describe an outbreak of NTED that occurred in a neonatal intensive care...

journal_title：Microbiology and immunology

authors： Nakano M,Miyazawa H,Kawano Y,Kawagishi M,Torii K,Hasegawa T,Iinuma Y,Ohta M

更新日期：2002-01-01 00:00:00

abstract：:Several liposomes containing ovalbumin (OVA), a model antigen, with different lipid compositions were prepared in order to evaluate their ability to induce oral tolerance. Oral administration of these liposomal OVAs induced suppression of the proliferative responses of popliteal lymph node cells from the treated mice ...

journal_title：Microbiology and immunology

authors： Masuda K,Horie K,Suzuki R,Yoshikawa T,Hirano K

更新日期：2002-01-01 00:00:00

abstract：:We elucidated the contribution of Valpha14 NKT cells to Th1 response and host resistance against mycobacterial infection. In Valpha14 NKT cell-deficient mice, host defense and DTH response to Mycobacterium bovis BCG were not different from wild-type mice after pulmonary infection. There was no significant difference i...

journal_title：Microbiology and immunology

authors： Kawakami K,Kinjo Y,Uezu K,Yara S,Miyagi K,Koguchi Y,Nakayama T,Taniguchi M,Saito A

更新日期：2002-01-01 00:00:00

abstract：:The present study was designed to evaluate the distribution of epithelioid cells, myofibroblasts, and TGF-beta1 in the formation of granuloma caused by Mycobacterium avium intracellulare complex (MAC) lung infection. A retrospective study was performed for 9 cases of positive MAC culture in which lung resections were ...

journal_title：Microbiology and immunology

authors： Fujita J,Ohtsuki Y,Suemitsu I,Yamadori I,Shigeto E,Shiode M,Nishimura K,Hirayama T,Matsushima T,Ishida T

更新日期：2002-01-01 00:00:00

abstract：:Human immunodeficiency virus type-1 (HIV-1) infection generally provokes antibody responses to the viral envelope glycoprotein. Two major regions of gp120, the third variable (V3) domain and the CD4-binding site, have been identified as neutralization targets. The precise mechanism of HIV-1 neutralization by antibodie...

journal_title：Microbiology and immunology

authors： Harada S

更新日期：2002-01-01 00:00:00

abstract：:Coagulase negative staphylococci (CNS) are significant pathogens, particularly in medical device related infections and in immunocompromised patients. Five CNS strains were isolated from 5 NOS2 knockout mice with dermatitis. Histologically, granulomatous dermatitis was found in the skin around the ear with epidermal u...

journal_title：Microbiology and immunology

authors： Won YS,Kwon HJ,Oh GT,Kim BH,Lee CH,Park YH,Hyun BH,Choi YK

更新日期：2002-01-01 00:00:00

abstract：:Antimicrobial peptides contribute to innate host defense against a number of bacteria and fungal pathogens. Some of antimicrobial synthetic peptides were systemically administered in vivo; however, effective protection has so far not been obtained because the effective dose of peptides in vivo seems to be very high, o...

journal_title：Microbiology and immunology

authors： Hamamoto K,Kida Y,Zhang Y,Shimizu T,Kuwano K

更新日期：2002-01-01 00:00:00

abstract：:During Helicobacter pylori infection, T cell response is critical in the development of active gastritis and in protective immunity against infection. We studied gastric inflammation and T cell response in H. pylori-challenged mice following an intraperitoneal immunization, using whole H. pylori lysate (HpAg) in the a...

journal_title：Microbiology and immunology

authors： Watanabe K,Murakami K,Maeda K,Fujioka T,Nasu M,Nishizono A

更新日期：2002-01-01 00:00:00

abstract：:We previously reported the establishment of an HEp2 cell line which expresses the US3 protein kinase (PK) of herpes simplex virus type 2 (HSV-2) upon induction with IPTG. Here we report that expression, phosphorylation and ubiquitination of cytokeratin 17 (CK17) are enhanced in US3-expressing HEp2 cells. In vitro kina...

journal_title：Microbiology and immunology

authors： Murata T,Goshima F,Nishizawa Y,Daikoku T,Takakuwa H,Ohtsuka K,Yoshikawa T,Nishiyama Y

更新日期：2002-01-01 00:00:00

abstract：:The protective role of neutrophils on intranasal infection of influenza virus was investigated in 3 strains of tumor-bearing mice with neutrophilic leukocytosis. In vitro multiplication of influenza virus was inhibited by neutrophils from both normal and tumor-bearing mice, and the inhibitory effect of neutrophils was...

journal_title：Microbiology and immunology

authors： Fujisawa H

更新日期：2001-01-01 00:00:00

abstract：:We constructed a plasmid containing bovine leukemia virus (BLV)-tax gene driven by SR alpha promoter, designated as pME-BLVtax, to activate the promoter of the long terminal repeat (LTR) of BLV in various tumor cells. Activation of the promoter of BLV-LTR by pME-BLVtax was confirmed by luciferase assay. When the cells...

journal_title：Microbiology and immunology

authors： Tana,Watarai S,Aida Y,Tajima S,Kakidani H,Onuma M,Kodama H

更新日期：2001-01-01 00:00:00

abstract：:Cross-linking of surface receptors in hematopoietic cells results in the enrichment of these receptors in the rafts along with other downstream signaling molecules. A possible explanation how signal is transduced through the plasma membrane has arisen from the concept of raft. From the study of cellular responses in t...

journal_title：Microbiology and immunology

authors： Katagiri YU,Kiyokawa N,Fujimoto J

更新日期：2001-01-01 00:00:00

abstract：:Intracellular localization of the wild-type (Spv+), the phoP-constitutively expressed strain (PhoPc), and the spv-deleted strain (Spv-) of Salmonella typhimurium was examined by the use of confocal laser scanning microscopy analysis of immunostained sections of mouse spleens after oral or subcutaneous inoculation. Onl...

journal_title：Microbiology and immunology

authors： Matsui H,Eguchi M,Kikuchi Y

更新日期：2001-01-01 00:00:00

abstract：:Mg2+-availability in Staphylococcus aureus cells decreased significantly with increasing NaCl concentration in growth media. Cells grew in a NaCl-free, Chelex resin-treated complex medium only if the medium was supplemented with 50 microM MgCl2, while, growth was limited when the medium was further supplemented with 1...

journal_title：Microbiology and immunology

authors： Ochiai T

更新日期：2001-01-01 00:00:00

abstract：:Deep-seated trichosporonosis caused by Trichosporon asahii has a high mortality rate and a very poor prognosis. New species-specific oligonucleotide primers for T. asahii were developed from a sequence analysis of rRNA genes that included the internal transcribed spacer regions. A nested PCR assay with specific primer...

journal_title：Microbiology and immunology

authors： Sugita T,Nakajima M,Ikeda R,Niki Y,Matsushima T,Shinoda T

更新日期：2001-01-01 00:00:00

abstract：:Bacteria of Stenotrophomonas maltophilia have been isolated with increasing frequency from the airways of cystic fibrosis (CF) patients, usually following P. aeruginosa infections, but their adherence to human epithelial respiratory cells has never been investigated. In this study, various S. maltophilia strains were ...

journal_title：Microbiology and immunology

authors： De Vidipó LA,De Marques EA,Puchelle E,Plotkowski MC

更新日期：2001-01-01 00:00:00

abstract：:Structural studies were carried out on an O-antigenic polysaccharide moiety derived from Porphyromonas circumdentaria NCTC 12469, a reference strain of Porphyromonas species. The polysaccharide chain was composed of D-glucose, D-galactose, N-acetyl-D-glucosamine, and N-acetyl-D-galactosamine in a molar ratio of 1:2:1:...

journal_title：Microbiology and immunology

authors： Matsuo K,Isogai E,Araki Y

更新日期：2001-01-01 00:00:00